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Effects of donor plant growth status and preculture temperature on isolated microspore culture ability in japonica rice (Oryza sativa L.)

J.H. Xie, M.W. Gao, Q.H. Cai, Z.Q. Liang and Q.Z. Xue
Cereal Research Communications
Vol. 24, No. 2 (1996), pp. 133-138
Published by: Akadémiai Kiadó
Stable URL: http://www.jstor.org/stable/23785221
Page Count: 6
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Effects of donor plant growth status and preculture temperature on isolated microspore culture ability in japonica rice (Oryza sativa L.)
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Abstract

The effects of donor plant growth status and preculture temperature on isolated microspore culture ability were investigated in japonica rice. Although the duration of collecting materials in the field could be extended by arranging earlier or later sowing time, donor plants sown only in normal and earlier season could grow well, and microspores of these plants had a good culture response. The plating efficiencies of the three batches of donor materials sown on different dates were 1.51, 1.45, 0.28 calli for each plated anther, respectively, and only calli derived from microspores of donor plants sown in the earlier and normal season had an ability to regenerate green plantlets. Moreover, the microspore culture efficiency could be improved by using alternative temperature during preculture stage. The preculture of rice microspores at 24°C for 3 days followed by 27°C for 2 days or 35°C for 12 h followed by 27°C for 4.5 days had a higher culture efficiency than that of precultured at a sustained temperature 27°C for 5 days. Besides, the culture efficiency was declined obviously if precultured at 35°C for 1 day at first.

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