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Differential expression of Lipoxygenase genes among durum wheat cultivars

Filomena Manna, Grazia Maria Borrelli, Domenica Rita Massardo, Klaus Wolf, Pietro Alifano, Luigi Del Giudice and Natale Di Fonzo
Cereal Research Communications
Vol. 26, No. 1 (1998), pp. 23-30
Published by: Akadémiai Kiadó
Stable URL: http://www.jstor.org/stable/23785244
Page Count: 8
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Differential expression of Lipoxygenase genes among durum wheat cultivars
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Abstract

Using as a template genomic DNA extracted from the cultivar Ofanto of durum wheat (Triticum turgidum var. durum Desf.), and as DNA primers two oligonucleotides spanning Lipoxygenase-1 (LOX-1) gene region, evolutionary conserved in different plants, we have amplified in a PCR reaction a 1200 bp fragment. This DNA fragment has been subcloned and used to prepare probes in Slot-blot experiments. Our aim was to detect the expression LOX gene(s), at level of RNA, in different cultivars of Durum wheat. Preliminary results indicates that there are significant variations in the expression of LOX gene(s) in the tested samples, and a good negative correlation of LOX-1 mRNA levels with both semolina β-carotene content and semolina yellow index of durm wheat varieties utilized. The physiological significance of this relation will be discussed.

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