You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
An Arabidopsis cyclin promoter region is active in transgenic maize plants
A. Peres, K. Nikovics, J. de Almeida-Engler, G. Engler, D. Inzé, A. Fehér and D. Dudits
Cereal Research Communications
Vol. 27, No. 3 (1999), pp. 223-230
Published by: Akadémiai Kiadó
Stable URL: http://www.jstor.org/stable/23786142
Page Count: 8
You can always find the topics here!Topics: Corn, Cyclins, Cell cycle, Transgenic plants, DNA, RNA, Plants, Embryonic cells, Cell division, Laboratory staining techniques
Were these topics helpful?See something inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
We have produced transgenic maize carrying Arabidopsis cyclin promoter and β-glucuronidase chimeric fusion (cycB1;1At::GUS) by direct DNA uptake into embryogenic cell suspension protoplasts to study the activity of this cyclin promoter in a heterologous cell system. The transgenic maize lines exhibiting different levels of reporter gene expression show that regulatory elements from cycB1;1At gene as a dicot promoter can function in maize. Expression patterns revealed by fluorimetric, as well as, histochemical GUS reporter enzymatic assays indicated cell division-dependent expression of the GUS gene driven by this cycB1;At;1 promoter with elevated activity in proliferating cells and meristems. Treatment of tissues with inhibitors of mitosis increased the activity of cycB1;1At promoter, that suggests a link between mitotic phase of cell division and activity of cycB1;1At regulatory elements in transgenic maize.
Cereal Research Communications © 1999 Akadémiai Kiadó