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An Arabidopsis cyclin promoter region is active in transgenic maize plants

A. Peres, K. Nikovics, J. de Almeida-Engler, G. Engler, D. Inzé, A. Fehér and D. Dudits
Cereal Research Communications
Vol. 27, No. 3 (1999), pp. 223-230
Published by: Akadémiai Kiadó
Stable URL: http://www.jstor.org/stable/23786142
Page Count: 8
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
An Arabidopsis cyclin promoter region is active in transgenic maize plants
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Abstract

We have produced transgenic maize carrying Arabidopsis cyclin promoter and β-glucuronidase chimeric fusion (cycB1;1At::GUS) by direct DNA uptake into embryogenic cell suspension protoplasts to study the activity of this cyclin promoter in a heterologous cell system. The transgenic maize lines exhibiting different levels of reporter gene expression show that regulatory elements from cycB1;1At gene as a dicot promoter can function in maize. Expression patterns revealed by fluorimetric, as well as, histochemical GUS reporter enzymatic assays indicated cell division-dependent expression of the GUS gene driven by this cycB1;At;1 promoter with elevated activity in proliferating cells and meristems. Treatment of tissues with inhibitors of mitosis increased the activity of cycB1;1At promoter, that suggests a link between mitotic phase of cell division and activity of cycB1;1At regulatory elements in transgenic maize.

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