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Primary Structure of the Gene Encoding Rat Preprosomatostatin
Marc R. Montminy, Richard H. Goodman, Sharon J. Horovitch and Joel F. Habener
Proceedings of the National Academy of Sciences of the United States of America
Vol. 81, No. 11, [Part 1: Biological Sciences] (Jun. 1, 1984), pp. 3337-3340
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/23829
Page Count: 4
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The somatostatins are peptides of 14 and 28 amino acids that are produced in a variety of endocrine and nonendocrine tissues. These peptides inhibit the secretion of many different pituitary, pancreatic, and gastrointestinal hormones. Previously, we have reported the isolation and nucleotide sequence of a cDNA derived from a rat medullary thyroid carcinoma that encoded preprosomatostatin, a 116-amino-acid precursor of somatostatin. We now report the structural characterization of the rat somatostatin gene isolated from recombinant bacteriophage libraries prepared from rat liver DNA. The gene spans 1.2 kilobases and is interrupted within the coding sequence of prosomatostatin by a single intron of 630 bases. A sequence characteristic of a Goldberg--Hogness promoter (``TATA'' box), T-T-T-A-A-A-A, is located 31 bases upstream from the transcriptional initiation site. A repetitive DNA sequence, highly reiterated in the rat genome, is located in the 5′ flanking region of the gene within 900 bases of the initiation site.
Proceedings of the National Academy of Sciences of the United States of America © 1984 National Academy of Sciences