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An Improved Method for Seed-Bank Analysis: Seedling Emergence After Removing the Soil by Sieving
G. N. J. Ter Heerdt, G. L. Verweij, R. M. Bekker and J. P. Bakker
Vol. 10, No. 1 (Feb., 1996), pp. 144-151
Published by: British Ecological Society
Stable URL: http://www.jstor.org/stable/2390273
Page Count: 8
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1. The seedling emergence method for assessing the size of the seed bank is improved by washing soil samples on a fine sieve and spreading the thus concentrated samples in a 3-5 mm thick layer on sterilized potting compost. 2. The method largely increases the number of seedlings that emerge as compared to unconcentrated samples. Hand-sorting afterwards shows that the germination rates vary between 81 and 100% of the viable seeds present. 3. Ninety-five per cent of the seedlings will emerge within 6 weeks using this method. 4. The method greatly reduces the greenhouse space needed and enables examination of large sample volumes.
Functional Ecology © 1996 British Ecological Society