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An Improved Method for Seed-Bank Analysis: Seedling Emergence After Removing the Soil by Sieving

G. N. J. Ter Heerdt, G. L. Verweij, R. M. Bekker and J. P. Bakker
Functional Ecology
Vol. 10, No. 1 (Feb., 1996), pp. 144-151
DOI: 10.2307/2390273
Stable URL: http://www.jstor.org/stable/2390273
Page Count: 8
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
An Improved Method for Seed-Bank Analysis: Seedling Emergence After Removing the Soil by Sieving
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Abstract

1. The seedling emergence method for assessing the size of the seed bank is improved by washing soil samples on a fine sieve and spreading the thus concentrated samples in a 3-5 mm thick layer on sterilized potting compost. 2. The method largely increases the number of seedlings that emerge as compared to unconcentrated samples. Hand-sorting afterwards shows that the germination rates vary between 81 and 100% of the viable seeds present. 3. Ninety-five per cent of the seedlings will emerge within 6 weeks using this method. 4. The method greatly reduces the greenhouse space needed and enables examination of large sample volumes.

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