Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

A soybean seed protein with carboxylate-binding activity

Sangeeta Dhaubhadel, Kuflom Kuflu, Maria Carmen Romero and Mark Gijzen
Journal of Experimental Botany
Vol. 56, No. 419 (September 2005), pp. 2335-2344
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/24035725
Page Count: 10
  • Download ($42.00)
  • Cite this Item
A soybean seed protein with carboxylate-binding activity
Preview not available

Abstract

The seed coat serves as a multifunctional organ with a role in protection and for the supply of nutrients to the embryo sac during development. The composition of the legume seed coat differs from other seed tissues in many ways including its protein composition. An abundant 24 kDa protein (SC24) has been purified and identified from soybean (Glycine max [L.] Merr) seed hulls. The corresponding cDNA and genomic DNA clones for SC24 were isolated and characterized, and expression patterns were determined. The deduced protein sequence of 219 amino acids included an N-terminal signal peptide. Transcripts encoding SC24 were present in the seed coat from 30 days after pollination (DAP) until maturity, but the protein was not detected until the final stages of seed maturation. In mature seeds, most of the SC24 protein was localized to the parenchyma and aleurone layers of the seed coat. The expression of SC24 was also induced in vegetative tissues by pathogen infection and by wounding. The SC24 protein bound to an affinity column containing an isophthalic acid ligand, and was eluted with 7 mM citrate. Polyclonal antibodies raised against recombinant SC24 cross-reacted with the seed coat peroxidase enzyme, suggesting that these two proteins may share an antigenic determinant. Overall, the results indicate that SC24 belongs to a novel class of plant defence proteins with carboxylate-binding activity.

Page Thumbnails

  • Thumbnail: Page 
[2335]
    [2335]
  • Thumbnail: Page 
2336
    2336
  • Thumbnail: Page 
2337
    2337
  • Thumbnail: Page 
2338
    2338
  • Thumbnail: Page 
2339
    2339
  • Thumbnail: Page 
2340
    2340
  • Thumbnail: Page 
2341
    2341
  • Thumbnail: Page 
2342
    2342
  • Thumbnail: Page 
2343
    2343
  • Thumbnail: Page 
2344
    2344