Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Identification of new gene expression regulators specifically expressed during plant seed maturation

Laurent Gutierrez, Geneviève Conejero, Mathieu Castelain, Stéphanie Guénin, Jean-Luc Verdeil, Brigitte Thomasset and Olivier Van Wuytswinkel
Journal of Experimental Botany
Vol. 57, No. 9 (2006), pp. 1919-1932
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/24036153
Page Count: 14
  • Download ($42.00)
  • Cite this Item
Identification of new gene expression regulators specifically expressed during plant seed maturation
Preview not available

Abstract

A cDNA-AFLP approach on Linum usitatissimum (flax) was used to identify genes specifically expressed during the seed maturation process. Among the 20 000 cDNA-AFLP tags produced, 486 were selected for their seed-specific expression during maturation. When compared with the publicly available databases, half of them presented some significant similarity with known plant sequences. The results obtained confirmed the accuracy of the approach as numerous genes previously described as being expressed exclusively in plant seeds were identified in this screen. The focus was on sequences similar to plant regulators involved in the control of gene expression, either at the transcriptional, post-transcriptional, or post-translational levels. Using a real-time RT-PCR approach, seed-specific expression kinetics were confirmed for 13 of these regulators that were never characterized for being expressed during seed maturation. Among these, a flax gene of the non-LEC1-like HAP3 family and a flax MYB factor were shown to be expressed in specialized tissues of flax embryo using an in situ hybridization approach. By expression kinetic comparison between these flax genes and their Arabidopsis counterparts, it was found that the new HAP3 gene should be related to a ubiquitous seed maturation mechanism, while a new MYB factor appears to be related to a more seed-specific maturation mechanism. These results demonstrate the utility of the flax database in not only identifying new genes expressed during seed maturation but also in being able to highlight the distinction between conserved and non-conserved seed maturation mechanisms.

Page Thumbnails

  • Thumbnail: Page 
[1919]
    [1919]
  • Thumbnail: Page 
1920
    1920
  • Thumbnail: Page 
1921
    1921
  • Thumbnail: Page 
1922
    1922
  • Thumbnail: Page 
1923
    1923
  • Thumbnail: Page 
1924
    1924
  • Thumbnail: Page 
1925
    1925
  • Thumbnail: Page 
1926
    1926
  • Thumbnail: Page 
1927
    1927
  • Thumbnail: Page 
1928
    1928
  • Thumbnail: Page 
1929
    1929
  • Thumbnail: Page 
1930
    1930
  • Thumbnail: Page 
1931
    1931
  • Thumbnail: Page 
1932
    1932