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Characterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm
Taiji Kawakatsu, Masayuki P. Yamamoto, Sakiko Hirose, Masahiro Yano and Fumio Takaiwa
Journal of Experimental Botany
Vol. 59, No. 15 (2008), pp. 4233-4245
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/24037699
Page Count: 13
You can always find the topics here!Topics: Rice, Endosperm, Genes, Plants, Transgenic plants, Antibodies, Genetics, Storage proteins, Buttocks, Spermatozoa
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A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the chromosomal glutelin gene cluster. The limited GluD-1 size polymorphism among the rice varieties is due to amino acid substitutions rather than to post-transcriptional modification. GluD-1 is maximally expressed in the starchy endosperm starting at 5 d after flowering (DAF) and increasing through 30 DAF, a major difference from the other glutelins which are primarily expressed in the subaleurone from 10–16 DAF. Only about 0.2 kb of the GluD-1 promoter was sufficient to confer inner starchy endosperm-specific expression. The 0.2 kb truncated GluD-1 promoter contains a bifactorial endosperm box consisting of a truncated GCN4 motif (TGA(G/C)TCA) and AAAG Prolamin box (P box), and ACGT and AACA motifs as cis-regulatory elements. Gel retardation assays and trans-activation experiments indicated that the truncated GCN4 and P box are specifically recognized by RISBZ1 b-ZIP and RPBF Dof activators in vitro, respectively, and are synergistically transactivated, indicating that combinatorial interactions of these motifs are involved in essential endosperm-specific regulation. Furthermore, deviation from the cognate GCN4 motif alters tissue-specific expression in the inner starchy endosperm to include other endosperm tissues.
Journal of Experimental Botany © 2008 Oxford University Press