Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

The role of vacuolar processing enzyme (VPE) from Nicotiana benthamiana in the elicitor-triggered hypersensitive response and stomatal closure

Huajian Zhang, Suomeng Dong, Meifang Wang, Wei Wang, Wenwen Song, Xianying Dou, Xiaobo Zheng and Zhengguang Zhang
Journal of Experimental Botany
Vol. 61, No. 13 (2010), pp. 3799-3812
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/24038876
Page Count: 14
  • Download ($42.00)
  • Cite this Item
Preview not available
Preview not available

Abstract

Elicitors/pathogen-associated molecular patterns (PAMPs) trigger the plant immune system, leading to rapid programmed cell death (hypersensitive response, HR) and stomatal closure. Previous reports have shown that the vacuolar processing enzyme (VPE), a cysteine proteinase responsible for the maturation of vacuolar proteins, has caspase-1-like activity and mediates TMV- and mycotoxin-induced cell death. The role of VPE from Nicotiana benthamiana in the response to three elicitors: bacterial harpin, fungal Nep1, and oomycete boehmerin, is described here. Single-silenced (NbVPE1a or NbVPE1b) and dual-silenced (NbVPE1a/1b) N. benthamiana plants were produced by virus-induced gene silencing. Although NbVPE silencing does not affect H 2 O 2 accumulation triggered by boehmerin, harpin, or Nep1, the HR is absent in NbVPE1a- and NbVPE1a/1b-silenced plants treated with harpin alone. However, NbVPE-silenced plants develop a normal HR after boehmerin and Nep1 treatment. These results suggest that harpin-triggered HR is VPE-dependent. Surprisingly, all gene-silenced plants show significantly impaired elicitor-induced stomatal closure and elicitor-promoted nitric oxide (NO) production in guard cells. Dual-silenced plants show increased elicitor-triggered AOS production in guard cells. The accumulation of transcripts associated with defence and cell redox is modified by VPE silencing in elicitor signalling. Overall, these results indicate that VPE from N. benthamiana functions not only in elicitor-induced HR, but also in elicitor-induced stomatal closure, suggesting that VPE may be involved in elicitor-triggered immunity.

Page Thumbnails

  • Thumbnail: Page 
[3799]
    [3799]
  • Thumbnail: Page 
3800
    3800
  • Thumbnail: Page 
3801
    3801
  • Thumbnail: Page 
3802
    3802
  • Thumbnail: Page 
3803
    3803
  • Thumbnail: Page 
3804
    3804
  • Thumbnail: Page 
3805
    3805
  • Thumbnail: Page 
3806
    3806
  • Thumbnail: Page 
3807
    3807
  • Thumbnail: Page 
3808
    3808
  • Thumbnail: Page 
3809
    3809
  • Thumbnail: Page 
3810
    3810
  • Thumbnail: Page 
3811
    3811
  • Thumbnail: Page 
3812
    3812