If you need an accessible version of this item please contact JSTOR User Support

Regulation of Bacterial DNA Supercoiling: Plasmid Linking Numbers Vary with Growth Temperature

Edward Goldstein and Karl Drlica
Proceedings of the National Academy of Sciences of the United States of America
Vol. 81, No. 13, [Part 1: Biological Sciences] (Jul. 1, 1984), pp. 4046-4050
Stable URL: http://www.jstor.org/stable/24134
Page Count: 5
  • Download PDF
  • Cite this Item

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If you need an accessible version of this item please contact JSTOR User Support
Regulation of Bacterial DNA Supercoiling: Plasmid Linking Numbers Vary with Growth Temperature
Preview not available

Abstract

The level of DNA supercoiling can be altered either by breaking-rejoining reactions that change the DNA linking number or by environmental changes that alter the helical pitch of DNA. In vitro, temperature changes alter helical pitch and, thus, supercoiling. We find that plasmids isolated from bacteria grown at different temperatures exhibit differences in DNA linking numbers. The differences in plasmid linking numbers offset the effect temperature is expected to have on supercoiling. These results are consistent with the hypothesis that fine control of DNA topology in bacterial cells is brought about by changes in linking number to maintain a constant value for supercoiling.

Page Thumbnails

  • Thumbnail: Page 
4044
    4044
  • Thumbnail: Page 
4045
    4045
  • Thumbnail: Page 
4046
    4046
  • Thumbnail: Page 
4047
    4047
  • Thumbnail: Page 
4048
    4048