You are not currently logged in.
Access your personal account or get JSTOR access through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Chain Breakage of Deoxyribonucleic Acid Following Treatment with Low Doses of Sulphur Mustard
D. J. R. Laurence
Proceedings of the Royal Society of London. Series A, Mathematical and Physical Sciences
Vol. 271, No. 1347 (Feb. 12, 1963), pp. 520-530
Published by: Royal Society
Stable URL: http://www.jstor.org/stable/2414430
Page Count: 11
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
The effect of low doses of mono- and difunctional sulphur mustards on the physical properties of DNA has been examined by viscosity and sedimentation methods. Alkylation produces no immediate effect on the shape or size of the DNA molecule. On incubation at 37 ⚬C and pH 7 the molecular weight of the alkylated molecules decreases slowly with no indication of a coiling up of the molecule. The rate of breakage is proportional to the extent of alkylation and constant with time over a period of 3 weeks, the rate for the difunctional mustard being significantly greater than for the monofunctional mustard. Various possible mechanisms are considered for the formation of breaks. It is possible that a break in one strand of the double helix leads to a break in the opposite strand during prolonged incubation. The effect of heat treatment and exposure to mild alkali was also investigated by viscosity measurements.
Proceedings of the Royal Society of London. Series A, Mathematical and Physical Sciences © 1963 Royal Society