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Acetylcholine Receptors

D. P. L. Green, R. Miledi, M. Perez De La Mora and Angela Vincent
Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
Vol. 270, No. 908, A Discussion on Excitable Membranes (Jun. 10, 1975), pp. 551-559
Published by: Royal Society
Stable URL: http://www.jstor.org/stable/2417352
Page Count: 9
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Acetylcholine Receptors
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Abstract

α-Bungarotoxin is one of a class of proteins, isolated from snake venoms, which antagonize the action of acetylcholine at vertebrate neuromuscular junctions and 'electroplaques' of electric fish. α-Bungarotoxin blocks acetylcholine action irreversibly and may be labelled with either 125I or 3H. This irreversible binding is used as the basis of an in vitro assay for acetylcholine receptors, whether in intact tissue, membrane fragments or solubilized preparations. Acetylcholine receptors from Torpedo and denervated skeletal muscle have been solubilized and substantially purified using affinity chromatography. The distribution of acetylcholine receptors in several tissues has been determined, and an auto-immune response, induced by injection of purified Torpedo receptors, has been studied.

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