Access

You are not currently logged in.

Access JSTOR through your library or other institution:

login

Log in through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Restriction Site Variation in the Chloroplast Genome of Sorghum (Poaceae)

Melvin R. Duvall and John F. Doebley
Systematic Botany
Vol. 15, No. 3 (Jul. - Sep., 1990), pp. 472-480
DOI: 10.2307/2419363
Stable URL: http://www.jstor.org/stable/2419363
Page Count: 9
  • Read Online (Free)
  • Download ($12.00)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Restriction Site Variation in the Chloroplast Genome of Sorghum (Poaceae)
Preview not available

Abstract

Restriction site variation was analyzed in the chloroplast (cp) DNA's of six species of Sorghum and one species each of Cleistachne and Zea. Each cpDNA was separately digested with 14 restriction enzymes and hybridized with six maize or sorghum cpDNA probes that together comprise approximately 75% of the plastid genome. Eighty-four restriction site mutations and two deletion mutations were observed among the cpDNA's of these species. These variable character mutations were used in phylogenetic analyses to construct rooted, most-parsimonious, phylogenetic trees on which confidence limits were placed by bootstrap analysis. The estimated number of nucleotide substitutions per site between each of the cpDNA's was also calculated. These analyses 1) reveal a relatively high level of sequence divergence within the genus Sorghum relative to that of other genera; 2) confirm that Sorghum sect. Sorghum is a monophyletic group; 3) cast doubt on the monophyly of sect. Parasorghum; and 4) suggest that the genus Sorghum may be either paraphyletic or polyphyletic. The fact that restriction site presence/absence in the plastid genome could be easily studied between genera as divergent as Zea and Sorghum suggests that analysis of variation in cpDNA could be used to construct a phylogeny for the entire tribe Andropogoneae.

Page Thumbnails

  • Thumbnail: Page 
472
    472
  • Thumbnail: Page 
473
    473
  • Thumbnail: Page 
474
    474
  • Thumbnail: Page 
475
    475
  • Thumbnail: Page 
476
    476
  • Thumbnail: Page 
477
    477
  • Thumbnail: Page 
478
    478
  • Thumbnail: Page 
479
    479
  • Thumbnail: Page 
480
    480