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Early Events of Vesicular-Arbuscular Mycorrhiza Formation on Ri T-DNA Transformed Roots

G. Becard and J. A. Fortin
The New Phytologist
Vol. 108, No. 2 (Feb., 1988), pp. 211-218
Published by: Wiley on behalf of the New Phytologist Trust
Stable URL: http://www.jstor.org/stable/2433339
Page Count: 8
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Early Events of Vesicular-Arbuscular Mycorrhiza Formation on Ri T-DNA Transformed Roots
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Abstract

An in vitro system using Ri T-DNA transformed roots and the vesicular-arbuscular mycorrhizal fungus Gigaspora margarita Becker & Hall has been developed to study the initial events of mycorrhiza formation. Sucrose, sodium and phosphorus were found to be critical components of the medium used to establish the dual culture. Using a single spore as inoculum it was consistently possible to obtain colonization of a preselected point on the root and to time the colonization process (within 5 days). Abundant viable and aseptic spores can be obtained. The system is especially appropriate for studying the triggering of the fungal biotrophy towards the root.

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