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Number of Stomata in Phaseolus Vulgaris Studied with the Analysis of Variance Technique

Hugh B. Smith
American Journal of Botany
Vol. 24, No. 6 (Jun., 1937), pp. 384-387
Stable URL: http://www.jstor.org/stable/2437039
Page Count: 4
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Number of Stomata in Phaseolus Vulgaris Studied with the Analysis of Variance Technique
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Abstract

Using the "analysis of variance" method for establishing a difference between two selections of beans, corresponding areas of comparable leaves were selected for study. For each leaf used at least 25 unit areas were studied at random in the upper epidermis and 50 unit areas in the lower epidermis. In this connection, unit area means 0.129 square millimeters in the upper epidermis and 0.033 square millimeters in the lower epidermis. Unless P for the difference was less than 0.036, the two selections were considered as differing no more than do plants within the same pure line. It was determined that the upper epidermis of the two selections of beans differed significantly in number of stomata per square millimeter of leaf surface, in size of ordinary epidermal cells, and in size of paired guard cells. The two selections differed in the lower epidermis in size of paired guard cells. P for the difference was in all cases less than 10-6.

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