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Neurospora V. A Synthetic Medium Favoring Sexual Reproduction

Mogens Westergaard and Herschel K. Mitchell
American Journal of Botany
Vol. 34, No. 10 (Dec., 1947), pp. 573-577
Stable URL: http://www.jstor.org/stable/2437339
Page Count: 5
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Neurospora V. A Synthetic Medium Favoring Sexual Reproduction
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Abstract

A synthetic medium has been devised for promotion of the sexual cycle in Neurospora. This medium contains the following ingredients: KNO3, 0.1 per cent; KH2PO4, 0.1 per cent; MgSO4, 0.05 per cent; CaCl2, 0.01 per cent; NaCl 0.01 per cent; biotin, 5 μ g. per liter; trace elements; sucrose, 2.0 per cent; agar, 1.5 per cent; adjusted to pH 6.5. For use in crosses between mutants this medium may be supplemented with yeast extract, malt extract, hydrolyzed casein, vitamin mixture or a combination of all four.

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