Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Culture of the Dinoflagellate Gymnodinium with Soil Extract

Beatrice Marcy Sweeney
American Journal of Botany
Vol. 38, No. 9 (Nov., 1951), pp. 669-677
Stable URL: http://www.jstor.org/stable/2437913
Page Count: 9
  • Read Online (Free)
  • Download ($12.00)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Culture of the Dinoflagellate Gymnodinium with Soil Extract
Preview not available

Abstract

The isolation and culture of an unarmoured dinoflagellate, tentatively identified as Gymnodinium splendens is described. This organism could not be subcultured indefinitely without the presence of a factor or factors present in extracts of garden soil. Soil extract was not active freshly prepared, but became active after aging for a period of 4-6 weeks in a sterile condition. Extracts lost activity after a period of 2-3 months but could be preserved by deep-freezing. Activity was also present in plasmoptysate of Prorocentrum micans cells and in sea water collected during a bloom of Gymnodinium. Ferric citrate, sodium citrate, Hoagland and Arnon's minor mineral nutrients, alanine, cystine, asparagine, a mixture of growth substances, peptone, dextrose, yeast extract, bean extract, and extracts of leaf mold and steer manure were all inactive at the concentrations assayed.

Page Thumbnails

  • Thumbnail: Page 
669
    669
  • Thumbnail: Page 
670
    670
  • Thumbnail: Page 
671
    671
  • Thumbnail: Page 
672
    672
  • Thumbnail: Page 
673
    673
  • Thumbnail: Page 
674
    674
  • Thumbnail: Page 
675
    675
  • Thumbnail: Page 
676
    676
  • Thumbnail: Page 
677
    677