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Culture of the Dinoflagellate Gymnodinium with Soil Extract
Beatrice Marcy Sweeney
American Journal of Botany
Vol. 38, No. 9 (Nov., 1951), pp. 669-677
Published by: Botanical Society of America, Inc.
Stable URL: http://www.jstor.org/stable/2437913
Page Count: 9
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The isolation and culture of an unarmoured dinoflagellate, tentatively identified as Gymnodinium splendens is described. This organism could not be subcultured indefinitely without the presence of a factor or factors present in extracts of garden soil. Soil extract was not active freshly prepared, but became active after aging for a period of 4-6 weeks in a sterile condition. Extracts lost activity after a period of 2-3 months but could be preserved by deep-freezing. Activity was also present in plasmoptysate of Prorocentrum micans cells and in sea water collected during a bloom of Gymnodinium. Ferric citrate, sodium citrate, Hoagland and Arnon's minor mineral nutrients, alanine, cystine, asparagine, a mixture of growth substances, peptone, dextrose, yeast extract, bean extract, and extracts of leaf mold and steer manure were all inactive at the concentrations assayed.
American Journal of Botany © 1951 Botanical Society of America, Inc.