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Separation and Mounting of Leaf Vein Skeletons and Epidermis
R. T. Whittenberger and J. Naghski
American Journal of Botany
Vol. 35, No. 10 (Dec., 1948), pp. 719-722
Published by: Botanical Society of America, Inc.
Stable URL: http://www.jstor.org/stable/2438152
Page Count: 4
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A brief fermentation of leaves with Clostridium roseum digests the mesophyll parenchyma cell walls sufficiently to permit the ready separation of intact epidermis and veins from the parenchyma protoplasts. The epidermis and even the most fragile vein skeletons are mounted easily on the emulsion surface of a photographic plate or printing paper. This gives permanent, durable, and undistorted specimens suitable for research and classroom study.
American Journal of Botany © 1948 Botanical Society of America, Inc.