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Chemical Control of Growth and Bud Formation in Tobacco Stem Segments and Callus Cultured in Vitro

Folke Skoog and Cheng Tsui
American Journal of Botany
Vol. 35, No. 10 (Dec., 1948), pp. 782-787
Stable URL: http://www.jstor.org/stable/2438159
Page Count: 6
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Chemical Control of Growth and Bud Formation in Tobacco Stem Segments and Callus Cultured in Vitro
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Abstract

Callus and stem internode segments of tobacco have been cultured in White's nutrient medium with and without addition of adenine, adenosine, alpha-naphthaleneacetic acid, increased phosphate and sucrose supplied singly and in mixtures. Under the conditions of the experiments adenine induces bud formation in both callus and stem internode tissues. NAA stimulates callus growth and root formation in stem segments, but it inhibits bud formation. Combinations of adenine and NAA greatly stimulate cell proliferation and enlargement of all tissues, particularly of the pith, but do not cause organ formation. Additional phosphate and sucrose enhance bud formation and tend to counteract the inhibiting effects of NAA on growth and organ formation in callus cultures. The results, therefore, demonstrate chemical control of organ formation and the type of growth of tissues within the limits of the experimental conditions. Relations between adenine and auxin in their effects on growth are discussed.

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