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Journal Article

Isolation, Growth, and Respiration of a Thermophilic Blue-Green Alga

Raymond W. Holton
American Journal of Botany
Vol. 49, No. 1 (Jan., 1962), pp. 1-6
Stable URL: http://www.jstor.org/stable/2439381
Page Count: 6

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Topics: Algae, Respiration, Plant growth, Flasks, Aeration, Cubes, Cyanobacteria, pH, Phosphates, Cell growth
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Isolation, Growth, and Respiration of a Thermophilic Blue-Green Alga
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Abstract

The isolation of a bacteria-free culture of Hapalosiphon laminosus (= Mastigocladus laminosus) from an algal mass obtained from Liard Hot Springs, British Columbia, is described. Seven different bacterial media at 2 incubation temperatures were used to test for bacteria in suspensions of partially broken up algae. The algal growth rate was obtained by calculating the slope of the straight line obtained in a plot of the cube root of the dry wt against time. In cultures aerated with air the growth was optimal between 35 and 50 C, with an upper limit at 55 and a lower limit at 25. Aeration with 1% CO2 in air significantly increased growth rates and the optimum was at 45-50 C. Unaerated flasks grew more slowly than aerated ones. The endogenous Qo2 (in μl/hr-mg dry wt) at 45 was 8.0 for growing cells and 2.8 for starved cells kept in darkness 18 hr. The Qo2 was maximal between pH 7.0 and 8.5. The concentration of phosphate buffer affected the Qo2 and rates in 0.02 M were slightly greater than those in distilled water and much greater than those in 0.4 M. The Qo2 of growing cells was slightly stimulated by 2,4-dinitrophenol (DNP), whereas glucose or glucose plus DNP did not affect it. In contrast, the endogenous Qo2 of starved cells was nearly doubled in glucose and tripled in DNP plus glucose.

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