Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If you need an accessible version of this item please contact JSTOR User Support

Histones and Alkaline Fast Green Staining of Onion Roots

Ernest M. Gifford, Jr. and Ronald E. Dengler
American Journal of Botany
Vol. 53, No. 10 (Nov. - Dec., 1966), pp. 1125-1132
Stable URL: http://www.jstor.org/stable/2440693
Page Count: 8
  • Read Online (Free)
  • Download ($12.00)
  • Cite this Item
If you need an accessible version of this item please contact JSTOR User Support
Histones and Alkaline Fast Green Staining of Onion Roots
Preview not available

Abstract

Onion root tips were freeze killed or fixed in 12 different chemical fixatives to determine the possible effects of such treatments on subsequent staining with alkaline Fast green (pH 8.0-8.1) to reveal the. presence of histones. Ethanol, methanol, neutral formalin, and a mixture of methanolchloroform-acetic acid produced nearly comparable results. Subsequent to fixation or freeze drying the sections were treated with hot 5% trichloroacetic acid (TCA) to remove nucleic acids prior to Fast green staining. Staining of histones does not occur in chemically fixed material if TCA hydrolysis is omitted. Nuclei, chromosomes during mitosis, and cytoplasm (to varying degrees) were stainable after TCA treatment. Positive staining occurs in nuclei, nucleoli, and cytoplasm in freeze-dried material with or without prior TCA treatment. CompariSons are made with chemical fixations. The use of ribonuclease instead of TCA permits staining of the cytoplasm and of chromosomes during mitosis. Discussions are included on the stainability of nucleoli and the possibility that native histone is associated with ribonucleic acid (RNA).

Page Thumbnails

  • Thumbnail: Page 
1125
    1125
  • Thumbnail: Page 
1126
    1126
  • Thumbnail: Page 
1127
    1127
  • Thumbnail: Page 
[1128]
    [1128]
  • Thumbnail: Page 
1129
    1129
  • Thumbnail: Page 
1130
    1130
  • Thumbnail: Page 
1131
    1131
  • Thumbnail: Page 
1132
    1132