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Shoot Tip Culture of Helianthus annuus-Flowering and Development of Adventitious and Multiple Shoots
Karol E. Paterson
American Journal of Botany
Vol. 71, No. 7 (Aug., 1984), pp. 925-931
Published by: Botanical Society of America, Inc.
Stable URL: http://www.jstor.org/stable/2443662
Page Count: 7
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Shoot multiplication of Helianthus annuus was optimal from half shoot apices cultured on MS media with 0.1-1.0 mg/l benzyl adenine or kinetin. Auxins inhibited multiplication and promoted callusing. Rooting was poor and was not promoted by auxins. Flowering of multiple shoots was observed after as little as 3 wk of culture. A number of plant growth regulators and environmental conditions had no effect on flowering which supports the determinate apex theory for sunflower (Habermann and Sekulow, 1972). Adventitious shoots were induced on leaves of the multiple shoots in some inbreds.
American Journal of Botany © 1984 Botanical Society of America, Inc.