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6(5)Carboxyfluorescein as a Tracer of Phloem Sap Translocation
Nicole Grignon, Bruno Touraine and Monique Durand
American Journal of Botany
Vol. 76, No. 6 (Jun., 1989), pp. 871-877
Published by: Botanical Society of America, Inc.
Stable URL: http://www.jstor.org/stable/2444542
Page Count: 7
You can always find the topics here!Topics: Phloem, Fluorescence, Petioles, Plants, Leaves, Soybeans, pH, Stems, Xylem, Wavelengths
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6(5)carboxyfluorescein (6(5)CF), a polar fluorescein with an apparent pK of 6.3, was introduced, as a pH 6.3 solution, into the apoplast of lamina or petioles of mature soybean leaves. Freehand sections were prepared at various times and immediately observed with a fluorescence microscope. 6(5)CF-associated fluorescence appeared in all sink organs, from shoot apex to roots. It was strictly confined to the phloem regions, even after 4 days. Its transport into young leaves ceased at approximately the time they underwent sink-to-source transition. It was never transported between two leaflets of the same leaf. Its transport was interrupted by phloem destruction. All these transport characteristics were highly reproducible, and were paralleled by those of 14C transport after application of (14C) sucrose to leaf surfaces. In contrast with 6(5)CF, fluorescein was transported between mature leaves, and between leaflets of the same leaf. It was not restricted to phloem, and often appeared in the xylem region. These results indicate that 6(5)CF can be used to monitor phloem sap translocation in real time, in short- and long-term experiments
American Journal of Botany © 1989 Botanical Society of America, Inc.