Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Rapid Screening Method to Detect Potential Biparental Inheritance of Plastid DNA and Results for Over 200 Angiosperm Species

Joseph L. Corriveau and Annette W. Coleman
American Journal of Botany
Vol. 75, No. 10 (Oct., 1988), pp. 1443-1458
Stable URL: http://www.jstor.org/stable/2444695
Page Count: 16
  • Read Online (Free)
  • Download ($12.00)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Rapid Screening Method to Detect Potential Biparental Inheritance of Plastid DNA and Results for Over 200 Angiosperm Species
Preview not available

Abstract

We have developed a diagnostic method to screen rapidly for plant species potentially capable of biparental inheritance of plastid DNA using the DNA fluorochrome 4',6-diamidino-2-phenyl-indole (DAPI) in conjunction with epifluorescence microscopy. Pollen shed from 235 plant species (including about 50 of agronomic importance) representing 80 families were screened. Putative plastid DNA was detected in the generative and/or sperm cells of pollen from 26 genera (43 species) representing 15 families. Plastid DNA was not detected in the generative or sperm cells of pollen from 192 plant species, thereby strongly suggesting that these species have only maternal inheritance. Our cytological diagnosis corroborated the known genetic evidence in 42 plant species and conflicted with the genetic reports in five species, which are discussed. The data suggest that biparental inheritance of plastids is rare; overall, it may occur in about 14% of flowering plant genera, examples of which are scattered among 19% of the families examined. This methodology also readily reveals whether pollen is bi- or trinucleate.

Page Thumbnails

  • Thumbnail: Page 
1443
    1443
  • Thumbnail: Page 
1444
    1444
  • Thumbnail: Page 
1445
    1445
  • Thumbnail: Page 
1446
    1446
  • Thumbnail: Page 
1447
    1447
  • Thumbnail: Page 
1448
    1448
  • Thumbnail: Page 
1449
    1449
  • Thumbnail: Page 
1450
    1450
  • Thumbnail: Page 
1451
    1451
  • Thumbnail: Page 
1452
    1452
  • Thumbnail: Page 
1453
    1453
  • Thumbnail: Page 
1454
    1454
  • Thumbnail: Page 
1455
    1455
  • Thumbnail: Page 
1456
    1456
  • Thumbnail: Page 
1457
    1457
  • Thumbnail: Page 
1458
    1458