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Isolation and Characterization of a cDNA Clone for the Complete Protein Coding Region of the δ Subunit of the Mouse Acetylcholine Receptor

Robert J. LaPolla, Katharine Mixter Mayne and Norman Davidson
Proceedings of the National Academy of Sciences of the United States of America
Vol. 81, No. 24, [Part 1: Biological Sciences] (Dec. 15, 1984), pp. 7970-7974
Stable URL: http://www.jstor.org/stable/24463
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Isolation and Characterization of a cDNA Clone for the Complete Protein Coding Region of the δ  Subunit of the Mouse Acetylcholine Receptor
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Abstract

A mouse cDNA clone has been isolated that contains the complete coding region of a protein highly homologous to the δ subunit of the Torpedo acetylcholine receptor (AcChoR). The cDNA library was constructed in the vector λ gt10 from membrane-associated poly(A)+ RNA from BC3H-1 mouse cells. Surprisingly, the δ clone was selected by hybridization with cDNA encoding the γ subunit of the Torpedo AcChoR. The nucleotide sequence of the mouse cDNA clone contains an open reading frame of 520 amino acids. This amino acid sequence exhibits 59% and 50% sequence homology to the Torpedo AcChoR δ and γ subunits, respectively. However, the mouse nucleotide sequence has several stretches of high homology with the Torpedo γ subunit cDNA, but not with δ . The mouse protein has the same general structural features as do the Torpedo subunits. It is encoded by a 3.3-kilobase mRNA. There is probably only one, but at most two, chromosomal genes coding for this or closely related sequences.

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