Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

The Tortoise and the Hare: Choosing between Noncoding Plastome and Nuclear Adh Sequences for Phylogeny Reconstruction in a Recently Diverged Plant Group

Randall L. Small, Julie A. Ryburn, Richard C. Cronn, Tosak Seelanan and Jonathan F. Wendel
American Journal of Botany
Vol. 85, No. 9 (Sep., 1998), pp. 1301-1315
Stable URL: http://www.jstor.org/stable/2446640
Page Count: 15
  • Read Online (Free)
  • Download ($12.00)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
The Tortoise and the Hare: Choosing between Noncoding Plastome and Nuclear Adh Sequences for Phylogeny Reconstruction in a Recently Diverged Plant Group
Preview not available

Abstract

Phylogenetic resolution is often low within groups of recently diverged taxa due to a paucity of phylogenetically informative characters. We tested the relative utility of seven noncoding cpDNA regions and a pair of homoeologous nuclear genes for resolving recent divergences, using tetraploid cottons (Gossypium) as a model system. The five tetraploid species of Gossypium are a monophyletic assemblage derived from an allopolyploidization event that probably occurred within the last 0.5-2 million years. Previous analysis of cpDNA restriction site data provided only partial resolution within this clade despite a large number of enzymes employed We sequenced three cpDNA introns (rpll6, rpoC1, ndhA) and four cpDNA spacers (accD-psal, trnL-trnF, trnT-trnL, atpB-rbcL) for a total of over 7 kb of sequence per taxon, yet obtained only four informative nucleotide substitutions (0.05%) resulting in incomplete phylogenetic resolution. In addition, we sequenced a 1.65-kb region of a homoeologous pair of nuclear-encoded alcohol dehydrogenase (Adh) genes. In contrast with the cpDNA sequence data, the Adh homoeologues yielded 25 informative characters (0 76%) and provided a robust and completely resolved topology that is concordant with previous cladistic and phenetic analyses. The enhanced resolution obtained using the nuclear genes reflects an approximately three- to sixfold increase in nucleotide substitution rate relative to the plastome spacers and introns.

Page Thumbnails

  • Thumbnail: Page 
1301
    1301
  • Thumbnail: Page 
1302
    1302
  • Thumbnail: Page 
1303
    1303
  • Thumbnail: Page 
1304
    1304
  • Thumbnail: Page 
1305
    1305
  • Thumbnail: Page 
1306
    1306
  • Thumbnail: Page 
1307
    1307
  • Thumbnail: Page 
1308
    1308
  • Thumbnail: Page 
1309
    1309
  • Thumbnail: Page 
1310
    1310
  • Thumbnail: Page 
1311
    1311
  • Thumbnail: Page 
1312
    1312
  • Thumbnail: Page 
1313
    1313
  • Thumbnail: Page 
1314
    1314
  • Thumbnail: Page 
1315
    1315