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Extension of Bacteriophage λ Host Range: Selection, Cloning, and Characterization of a Constitutive λ Receptor Gene
Gert E. de Vries, Chris K. Raymond and Robert A. Ludwig
Proceedings of the National Academy of Sciences of the United States of America
Vol. 81, No. 19, [Part 1: Biological Sciences] (Oct. 1, 1984), pp. 6080-6084
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/24567
Page Count: 5
You can always find the topics here!Topics: Bacteriophages, Plasmids, Bacteria, Cosmids, Lambs, Phenotypes, Receptors, Adsorption, Colony forming units assay, Cells
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A set of plasmids has been constructed that carry a constitutive lamB gene (LamBc phenotype) from Escherichia coli and that confer functional phage λ receptors to bacteria other than E. coli. This E. coli LamBc strain has been selected to escape both maltose-inducible and glucose-repressible control. Constitutivity results from an IS-3 insertion, carrying a mobile promoter, proximal to lamB. The LamBc DNA has been cloned into both broad and narrow host-range plasmids, and the resulting pTROY plasmids have been transferred to diverse bacteria. Both Salmonella typhimurium/pTROY and Klebsiella pneumoniae/pTROY strains efficiently adsorb phage λ ; Pseudomonas aeruginosa/pTROY strains do not. Introduction of a functional E. coli LamB protein into foreign bacteria will allow these bacteria carrying pTROY plasmids to be infected by phage λ recombinant DNA libraries, phage λ:: Tn insertion mutagenesis vectors, and in vivo λ -packaged cosmids.
Proceedings of the National Academy of Sciences of the United States of America © 1984 National Academy of Sciences