Nonsymbiotic Germination of Orchid Seeds

Lewis Knudson
Botanical Gazette
Vol. 73, No. 1 (Jan., 1922), pp. 1-25
Stable URL:
Page Count: 25
  • Download PDF
  • Cite this Item

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:


Log in to your personal account or through your institution.

Nonsymbiotic Germination of Orchid Seeds


1. A method is given for sterilizing seeds of certain orchids and for growing them under sterile conditions. 2. Germination of seeds of Laelia, Cattleya, and related forms is possible without the aid of any fungus when certain sugars are supplied. 3. Fructose appears more favorable than glucose. 4. In the presence of glucose, chlorosis of the embryo generally results. 5. Germination is possible on certain plant extracts containing merely traces of sugar. 6. Embryos in sugar-containing cultures accumulate a considerable reserve of starch. 7. The concentration of glucose is important in the growth of the embryo. 8. Bacillus radicicola from alfalfa and certain other microorganisms on certain media have a favorable influence on the development of chlorophyll and germination. 9. Seedlings have been transplanted from tubes to large flasks and growth has continued. 10. The results thus far obtained indicate that the method is of value in the propagation of orchids from seeds. 11. The idea is advanced that the necessity of fungus infection for germination has not yet been proved. 12. One cause of failure of germination may be the pathogenic character of some of the endophytic fungi.

Notes and References

This item contains 13 references.

Literature Cited
  • 1
    BACHMANN, F. M., Vitamine requirements of certain yeasts. Jour. Biol. Chem.39:235. 1919.
  • 2
    BERNARD, NOEL, L' evolution dans la symbiose, les Orchidees et leur champignons commensaux. Ann. Sci. Nat. Bot.9: 1-i96. 1909.
  • 3
    BOTTOMLEY, W. B., The effect of nitrogen-fixing organisms and nucleic acid derivatives on plant growth. Roy. Soc. London Proc. B91:83-95. 1919.
  • 4
    BURGEFF, HANS, Die Wurzelpilze der Orchideen, ihre Kultur und ihre Leben in der Pflanze. Jena. 1909.
  • 5
    DUGGAR, B. M., SEVERY, J. W., and SCHMITZ, H., Studies in the physi- ology of fungi. IV. The growth of certain fungi in plant decoctions. Ann. Mo. Bot. Gard.4: 165-I73. 1917.
  • 6
    KING, JOHN, King's American Dispensatory, re-written and enlarged by H. W. FELTER and J. W. LLOYD, 18th ed., 3d revision, 2:1898 (p. 1699).
  • 7
    KNUDSON, L., Influence of certain carbohydrates on green plants. Cornell Univ. Agric. Exp. Sta. Memoir9:1-75. 1916.
  • 8
    KNUDSON, L., and LINDSTROM, E. W., Influence of sugars on the growth of albino plants. Amer. Jour. Bot.6:401-405. 1919.
  • 9
    MAZÉ, P., and PERRIER, A., Recherches sur l'assimilation de quelques substances ternaires par les vegetaux a chlorophylle. Inst. Pasteur Ann. 18:721-747. 1904.
  • 10
    SERVETTAZ, CAMILLE, Recherches experimentales sur la developpement et la nutrition des mousses en milieux sterilises. Ann. Sci. Nat. Bot. IX. 17:111-224. 1913.
  • 11
    WILLAMAN, J. J., The function of vitamines in the metabolism of Scleroti- nia cinerea. Jour. Amer. Chem. Soc.42:549-58 5. 1920.
  • 12
    WILLIAMS, The vitamine requirements of yeasts. Jour. Biol. Chem. 38:465-486. 1919.
  • 13
    WILSON, J. K., Calcium hypochlorite as a seed sterilizer. Amer. Jour. Bot.2:420-427. 1915.