Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Effect of Direct Current on Cells of Root Tip of Canada Field Pea

Henry F. A. Meier
Botanical Gazette
Vol. 72, No. 3 (Sep., 1921), pp. 113-138
Stable URL: http://www.jstor.org/stable/2470349
Page Count: 28
Subjects: Biological Sciences Environmental Science Botany & Plant Sciences
  • Download PDF
  • Add to My Lists
  • Cite this Item
Effect of Direct Current on Cells of Root Tip of Canada Field Pea
We're having trouble loading this content. Download PDF instead.

Abstract

1. A method of subjecting roots in moist air to the direct electric current has been devised which makes it possible to control and accurately measure the current actually flowing through the root. There is no evidence that under such conditions roots behave differently from those in soil or liquid if subjected to the same current intensity. 2. Combinations of current intensity and time factors have been determined for producing death of the cells of roots, and the death curve plotted. 3. Cytological preparations of treated roots show a migration of cell contents (with few exceptions) toward the positive electrode. 4. The migratory effect (transfer of material) is not the same for all regions of the root. With one-tenth of time of death current, the cells immediately back of the root cap show little effect, those a little older (1 mm. back) greatest effect, and the cells with large vacuoles no or little effect as to cytoplasm. 5. It is suggested that, with addition of water, more free ions may occur to conduct the current; that the protoplasts of the primordial meristem are in a state of gel. 6. It is further suggested that the difference in true acidity, H-ion concentration of various protoplasts, may account for occasional different behavior of adjacent cells. 7. The theory of electrophoresis probably accounts for the migration phenomena, assuming that the constituent colloidal particles of protoplasm bear an electric charge. 8. Assuming an electric charge carried by such particles, it would follow that the particles of the cytoplasm of the cells of the roots of the Canada Field Pea bear a negative charge, and that the chromatin particles, in some cases only, may bear a positive charge.

Notes and References

This item contains 22 references.

Literature Cited
  • 1
    AMICI, G. B., Osservazioni sulla Circulazione del Succhionella Citara. Mem. Matem. Soc. Ital. VIII.2: Med. 1818 (quoted from BECQUEREL).
  • 2
    BECQUEREL, A. C., Influence de 1e'lectricite sur la circulation du Chara. Compt. Rend.5:784-788. 1837.
  • 3
    CARLGREN, O., lber die Einwirkung des constanten galvanischen Stromes auf niedere Organismen. Archiv Anat. und Physiol.1900 (pp. 49-75).
  • 4
    CHAMBERS, R., Changes in protoplasmic consistency and their relation to cell division. Jour. Gen. Physiol.2:49-68. 1919.
  • 5
    COEHN, A., tMber ein Gesetz der Electricitatserregung. Ann. Physik und Chemie64:217-232. 1898.
  • 6
    DALE, H. H., Galvanotaxis and chemotaxis of ciliate infusoria. Jour. Physiol.26:291-36I. 1911.
  • 7
    This reference contains 2 citations:
    • Du BOIS-REYMOND, E., iVber die innere Polarisation por6ser mit Elektro- lyten getrankter Halbleiter. Berlin Akad. Wiss.1856 (pp. 450-468)
    • 1860 (pp. 846-906).
  • 8
    HARDY, W. B., The coagulation of proteid by electricity. Jour. Physiol. 24:288-304. 1899.
  • 9
    —, Note on difference in electrical potential within the living cell. Jour. Physiol.47:108-III. 1913.
  • 10
    This reference contains 2 citations:
    • HELMHOLTZ, H. VON, Studien uber elektrische Grenzschichten. WIEDE- MANN'S Annalen7:337. 1879
    • Mem. Physical Soc. London1 :1-42. 1888.
  • 11
    JURGENSEN, T., tVber die Bewegung fester, in FlUssigkeiten suspendierter Korper unter dem Einfluss des elektrischen Stromes. REIcIIIERT U1(d DU BoIs-REYMOND Archiv. Anat. und Physiol.1860 (pp. 673-687).
  • 12
    —, Uber die in den Zellen der Vallisneria spiralis stattfindenden Bewegungserscheinungen. Studien Physiol. Inst. Breslau1861 (pp. 87- 109).
  • 13
    KITE, G. L., Studies in the physical properties of protoplasm. Amer. Jour. I'hysiol.32:146-I64. 1913.
  • 14
    KÜHNE, W., Untersuchungen über das Protoplasma und die Contractilität. 1864.
  • 15
    LILLIE, R. S., On difference in the direction of the electrical convection of certain free cells and nuclei. Amer. Jour. Physiol.8:273-283. 1903.
  • 16
    MOTTIER, D. M., The effect of centrifugal force upon the cell. Ann. Botany13:325-360. 1899.
  • 17
    PERRIN, J. (Clectricite)-"Examen des conditions qui determinent le signe et la grandeur de l'osmose electrique et de l'electrisation par contact." (Note de 2M. JEAN PERRIN, presentee par MA. 2MASCART.) Compt. Rend. 136:1388-I391. 1903.
  • 18
    This reference contains 4 citations:
    • PICTON, H., and LINDER, S. E., Solution and pseudo-solution. Jour. Chem. Soc.61:148. 1892
    • 67:63. 1895
    • 71:568. 1897
    • 87:1906. I905.
  • 19
    QUINCKE,, G., tber die Fortfuhrung inaterieller Theilchen durchstr6men- der Elektricitat. Pogg. Annalen IV.23:513-598. 1861.
  • 20
    REUSS, F. F., Mein. Soc. Imp. Nat. Moscou2:327. 1809 (quoted by JURGENSEN).
  • 21
    This reference contains 4 citations:
    • VERWORN, M., Die polar Erregung der Protisten durch den galvanischen Strom. PFLUGER'S Archiv.5:1-36. 1889
    • 46:267-303. 1890
    • 62:415- 450. 1896
    • 65:47-62. 1897.
  • 22
    WIEDEMANN, G., Utber die Bewegung im Kreise der geschlossenen galva- nischen Saule. Pogg. Annalen87:321-352. 1852.