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Enhanced Recombination between λ plac5 and F42lac: Identification of cis- and trans-Acting Factors
H. Steven Seifert and Ronald D. Porter
Proceedings of the National Academy of Sciences of the United States of America
Vol. 81, No. 23, [Part 1: Biological Sciences] (Dec. 1, 1984), pp. 7500-7504
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/24735
Page Count: 5
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Enhanced transductional recombination between specialized transducing phage λ plac5 and plasmid F42lac depends on cis- and trans-acting factors. By constructing a series of recombinant molecules, the cis-acting site required for enhanced recombination has been identified as oriT, the origin of conjugational transfer of the F sex factor of Escherichia coli. The trans-acting factors are located in the promoter-proximal and the promoter-distal regions of the traY-to-traZ operon.
Proceedings of the National Academy of Sciences of the United States of America © 1984 National Academy of Sciences