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"Budding" and Nuclear Division in Cultured Protoplasts of Corn, Convolvulus, and Onion
S. Bala Bawa and John G. Torrey
Vol. 132, No. 3 (Sep., 1971), pp. 240-245
Published by: The University of Chicago Press
Stable URL: http://www.jstor.org/stable/2474100
Page Count: 6
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Protoplasts were isolated from seedlings of Zea mays L, roots of Convolvulus arvensis L, and root callus of C arvensis L. (filamentous strain of root callus) and Allium cepa L. by cellulase treatment They were maintained for over 3 weeks in liquid medium containing either 0.4 M sorbitol or a mixture of sorbitol and 1% sucrose. Protoplasts of C. arvensis L were observed to form protuberances or "buds"; such protoplasts demonstrated regenerated wall formation within 2-3 days of culture A few protoplasts exhibited nuclear divisions on the second day of culture About 4%-5% became binucleate, and 2% became three- to four-nucleate In experiments on the effect of "Sendai" virus on plant protoplasts, no agglutination or fusion was observed
Botanical Gazette © 1971 The University of Chicago Press