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The Metabolism of Species of Streptomyces. II. The Nitrate Metabolism of S. coelicolor

Vincent W. Cochrane and Jean E. Conn
Bulletin of the Torrey Botanical Club
Vol. 77, No. 1 (Jan. - Feb., 1950), pp. 10-18
Published by: Torrey Botanical Society
DOI: 10.2307/2482377
Stable URL: http://www.jstor.org/stable/2482377
Page Count: 9
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The Metabolism of Species of Streptomyces. II. The Nitrate Metabolism of S. coelicolor
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Abstract

Streptomyces coelicolor is able to utilize ammonium aspartate, asparagine, urea, casamino acids, and nitrate as sources of nitrogen, but maximum growth is obtained in a poorly buffered glucose medium only with the first two materials named. Growth inhibition in a glucose-nitrate medium results from the interaction of two metabolic systems, glucose dissimilation and nitrate reduction. At the Iow pH accompanying the formation of organic acids from glucose the nitrite produced from nitrate is toxic. The symptoms of toxicity are removed, and growth is normal, if the pPI of such a medium is maintained at 6.8 or higher. In media having as carbon source glycerol or mannitol, metabolic acids do not accumulate and growth on nitrate is satisfactory. Yeast extract accelerates growth on nitrate media, possibly by provision of compounds essential for the reduction of nitrate. The time course of growth on a neutralized glucose-nitrate medium with yeast extract includes a long lag period which may represent the time necessary either for the formation of adaptive enzymes or for the accumulation of oxidizable metabolites needed for the reduction of nitrate

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