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Embryoids and Complete Plants from Unpollinated Ovaries and from Ovules of in vivo-Grown Emasculated Flower Buds of Citrus spp.
G. C. Mitra and H. C. Chaturvedi
Bulletin of the Torrey Botanical Club
Vol. 99, No. 4 (Jul. - Aug., 1972), pp. 184-189
Published by: Torrey Botanical Society
Stable URL: http://www.jstor.org/stable/2484573
Page Count: 6
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Explants of unpollinated ovaries of Citrus aurantifolia and C. sinensis, but not of C. maxima, a monoembryonic species, produced embryoids and complete plants under certain cultural conditions. Subsequent experiments showed that ovary wall tissue was more prone to produce these responses. In long-term culture the responses gradually decreased resulting in complete cessation of embryoid formation, this cessation occurring earlier in C. aurantifolia than in C. sinensis. Explants of nucellus contained in longitudinal halves of ovules taken from in vivo-grown emasculated flower buds of C. aurantifolia produced three types of responses, namely, (a) an increasing mass of proliferating embryoids, (b) friable, spongy and pale-white tissue along with callusing integumentary tissue and (c) mixed responses of (a) and (b). The explants with continuously increasing mass of proliferating embryoids contained most of the stages of zygotic embryogenesis. Using different culture media, a good number of embryoids could be induced in explants of friable and spongy tissue of nucellar origin, but they did not develop into seedlings. No embryoids could be induced from callusing integumentary tissue regardless of medium employed.
Bulletin of the Torrey Botanical Club © 1972 Torrey Botanical Society