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Drosophila DNA Topoisomerase I is Associated with Transcriptionally Active Regions of the Genome
Gerhard Fleischmann, Gert Pflugfelder, Elizabeth K. Steiner, Kashayar Javaherian, Gary C. Howard, James C. Wang and Sarah C. R. Elgin
Proceedings of the National Academy of Sciences of the United States of America
Vol. 81, No. 22, [Part 1: Biological Sciences] (Nov. 15, 1984), pp. 6958-6962
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/24928
Page Count: 5
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The distribution of DNA topoisomerase I within Drosophila polytene chromosomes was observed by immunofluorescent staining with affinity-purified antibodies. The enzyme is preferentially associated with active loci, as shown by prominent staining of puffs. The heat shock loci 87A-87C are stained after, but not before, heat shock induction. A detailed comparison of the distribution of topoisomerase I with that of RNA polymerase II reveals a similar, although not identical, pattern of association. Topoisomerase I is also found in association with the nucleolus, the site of transcription by RNA polymerase I.
Proceedings of the National Academy of Sciences of the United States of America © 1984 National Academy of Sciences