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# Phosphate Removal by Algal Systems

Nicholas E. Vanderborgh and Archie G. Buyers
Journal (Water Pollution Control Federation)
Vol. 46, No. 4 (Apr., 1974), pp. 726-734
Stable URL: http://www.jstor.org/stable/25038186
Page Count: 9
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## Abstract

The kinetics of the radioactive isotope ${}^{32}{\rm P}$ uptake by the freshwater algae Chlorella have been investigated in soil-water cultures and synthetic culture media at three pH values and at 20° ± 2°C. Reagent grade sodium pyrophosphate was irradiated with fast-neutron flux converting a portion of the sample into the radioisotope ${}^{32}{\rm P}$, producing an activity of 0.33 μc/g. Tagged pyrophosphate solutions were used to spike Chlorella cultures. The uptake of the isotope was monitored as a function of time. The kinetic interpretation must allow for first-order kinetics with respect to ${}^{32}{\rm P}$ at a pH of 10 and a second-order kinetic scheme at pH 4 and 7. The kinetic evidence indicates that this algal system can only utilize phosphorus in the orthophosphate form.

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