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Evidence for Linear Response for the Induction of Mutations in Human Cells by X-Ray Exposures below 10 Rads
Andrew J. Grosovsky and John B. Little
Proceedings of the National Academy of Sciences of the United States of America
Vol. 82, No. 7 (Apr. 1, 1985), pp. 2092-2095
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/25041
Page Count: 4
You can always find the topics here!Topics: Radiation dosage, Dose response relationship, Radiation dose response relationship, Irradiation, Linear regression, Mutagenesis, Genetic mutation, Cultured cells, Somatic cells, Diploidy
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The induction of 6-thioguanine resistance (6TGR) was studied in continuous human lymphoblast cultures exposed to daily x-ray doses of 1, 2.5, 5, or 10 rads (1 rad = 0.01 gray) for periods up to 1 month. Samples were taken every 5 days for determinations of induced mutation frequency. Cells receiving daily doses of 1-10 rads showed a mutation frequency of 0.069 × 10-6 6TGR cell per rad, virtually identical to the value of 0.062 × 10-6 6TGR cell per rad determined for lymphoblasts receiving the same total dose of radiation in a single acute exposure. The effects of small daily fractions were additive, suggesting that doses as small as 1 rad are mutagenic in human lymphoblasts. Similar results were observed when resistance to trifluorothymidine, indicative of thymidine kinase deficiency, was used as a mutational marker. When 6TGR frequency was plotted against days of irradiation, a positive linear slope was observed for all dose groups. The values of these slopes were plotted against x-ray dose in rads/day to construct a dose-response relationship for 1-10 rads. A linear increase in mutation frequency was observed over this dose range, with no apparent threshold. The slope of this linear increase was 0.060 × 10-6 6TGR cell per rad. These results suggest that, for human lymphoblasts, the mutagenic risk of low doses of x-rays can be accurately estimated by linear extrapolation from high-dose effects.
Proceedings of the National Academy of Sciences of the United States of America © 1985 National Academy of Sciences