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# Successful Extraction of DNA from 100-Year-Old Herbarium Specimens of the Liverwort Bazzania trilobata

Kamila Jankowiak, Katarzyna Buczkowska and Zofia Szweykowska-Kulinska
Taxon
Vol. 54, No. 2 (May, 2005), pp. 335-336
DOI: 10.2307/25065361
Stable URL: http://www.jstor.org/stable/25065361
Page Count: 2
Using a modified DNeasy™ Plant Mini Kit method to extract DNA, we were able to isolate DNA from 100-year-old liverwort herbarium specimens. We sucessfully isolated DNA from 18 specimens of the liverwort Bazzania trilobata collected during the $20^{{\rm th}}$ century. Our observations suggest that, at least over 100 years of material storage, the most crucial factor in successful DNA isolation is method of drying, not age of sample.