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Microgenomic Analysis Reveals Cell Type-Specific Gene Expression Patterns between Ray and Fusiform Initials within the Cambial Meristem of Populus

Nadia Goué, Marie-Claude Lesage-Descauses, Ewa J. Mellerowicz, Elisabeth Magel, Philippe Label and Björn Sundberg
The New Phytologist
Vol. 180, No. 1 (Oct., 2008), pp. 45-56
Published by: Wiley on behalf of the New Phytologist Trust
Stable URL: http://www.jstor.org/stable/25150550
Page Count: 12
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Microgenomic Analysis Reveals Cell Type-Specific Gene Expression Patterns between Ray and Fusiform Initials within the Cambial Meristem of Populus
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Abstract

The vascular cambium is the meristem in trees that produce wood. This meristem consists of two types of neighbouring initials: fusiform cambial cells (FCCs), which give rise to the axial cell system (i.e. fibres and vessel elements), and ray cambial cells (RCCs), which give rise to rays. There is little molecular information on the mechanisms whereby the differing characteristics of these neighbouring cells are maintained. A microgenomic approach was adopted in which the transcriptomes of FCCs and RCCs dissected out from the cambial meristem of poplar (Populus trichocarpa × Populus deltoïdes var. Boelare) were analysed, and a transcriptional database for these two cell types established. Photosynthesis genes were overrepresented in RCCs, providing molecular support for the presence of photosynthetic systems in rays. Genes that putatively encode transporters (vesicle, lipid and metal ion transporters and aquaporins) in RCCs were also identified. In addition, many cell wall-related genes showed cell type-specific expression patterns. Notably, genes involved in pectin metabolism and xyloglucan metabolism were overrepresented in RCCs and FCCs, respectively. The results demonstrate the use of microgenomics to reveal differences in biological processes in neighbouring meristematic cells, and to identify key genes involved in these processes.

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