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Consensus Sequence for Escherichia coli Heat Shock Gene Promoters
Deborah W. Cowing, James C. A. Bardwell, Elizabeth A. Craig, Carol Woolford, Roger W. Hendrix and Carol A. Gross
Proceedings of the National Academy of Sciences of the United States of America
Vol. 82, No. 9 (May 1, 1985), pp. 2679-2683
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/25240
Page Count: 5
You can always find the topics here!Topics: Promoter regions, RNA, Shock heating, Genes, DNA probes, RNA probes, Consensus sequence, Heat shock proteins, Transfer RNA, Plasmids
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We have identified promoters for the Escherichia coli heat shock operons dnaK and groE and the gene encoding heat shock protein C62.5. Transcription from each promoter is heat-inducible in vivo, and each is recognized in vitro by RNA polymerase containing σ 32, the σ factor encoded by rpoH (htpR) but not by RNA polymerase containing σ 70. We compared the sequences of the heat shock promoters and propose a consensus promoter sequence, having T-N-t-C-N-C-c-C-T-T-G-A-A in the -35 region and C-C-C-C-A-T-t-T-a in the -10 region. These sequences differ from the consensus sequence recognized by holoenzyme containing σ 70, the major σ in E. coli. We suggest that the accumulated consensus sequences of promoters recognized by alternate forms of holoenzyme are compatible with a model in which σ recognizes only the -10 region of the promoter.
Proceedings of the National Academy of Sciences of the United States of America © 1985 National Academy of Sciences