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The Shoot Meristem Identity Gene TFL1 Is Involved in Flower Development and Trafficking to the Protein Storage Vacuole
Eun Ju Sohn, Marcela Rojas-Pierce, Songqin Pan, Clay Carter, Antonio Serrano-Mislata, Francisco Madueño, Enrique Rojo, Marci Surpin and Natasha V. Raikhel
Proceedings of the National Academy of Sciences of the United States of America
Vol. 104, No. 47 (Nov. 20, 2007), pp. 18801-18806
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/25450505
Page Count: 6
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Plants are unique in their ability to store proteins in specialized protein storage vacuoles (PSVs) within seeds and vegetative tissues. Although plants use PSV proteins during germination, before photosynthesis is fully functional, the roles of PSVs in adult vegetative tissues are not understood. Trafficking pathways to PSVs and lytic vacuoles appear to be distinct. Lytic vacuoles are analogous evolutionarily to yeast and mammalian lysosomes. However, it is unclear whether trafficking to PSVs has any analogy to pathways in yeast or mammals, nor is PSV ultrastructure known in Arabidopsis vegetative tissue. Therefore, alternative approaches are required to identify components of this pathway. Here, we show that an Arabidopsis thaliana mutant that disrupts PSV trafficking identified TERMINAL FLOWER 1 (TFL1), a shoot meristem identity gene. The tfl1-19/mtv5 (for "modified traffic to the vacuole") mutant is specifically defective in trafficking of proteins to the PSV. TFL1 localizes to endomembrane compartments and colocalizes with the putative δ-subunit of the AP-3 adapter complex. Our results suggest a developmental role for the PSV in vegetative tissues.
Proceedings of the National Academy of Sciences of the United States of America © 2007 National Academy of Sciences