You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Protein Evolution with an Expanded Genetic Code
Chang C. Liu, Antha V. Mack, Meng-Lin Tsao, Jeremy H. Mills, Hyun Soo Lee, Hyeryun Choe, Michael Farzan, Peter G. Schultz and Vaughn V. Smider
Proceedings of the National Academy of Sciences of the United States of America
Vol. 105, No. 46 (Nov. 18, 2008), pp. 17688-17693
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/25465345
Page Count: 6
You can always find the topics here!Topics: Bacteriophages, Amino acids, Antibodies, Libraries, Evolution, Codons, Genetic code, Directed molecular evolution, Plasmids, Enzyme linked immunosorbent assay
Were these topics helpful?See somethings inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
We have devised a phage display system in which an expanded genetic code is available for directed evolution. This system allows selection to yield proteins containing unnatural amino acids should such sequences functionally outperform ones containing only the 20 canonical amino acids. We have optimized this system for use with several unnatural amino acids and provide a demonstration of its utility through the selection of anti-gp120 antibodies. One such phage-displayed antibody, selected from a naïve germline scFv antibody library in which six residues in VH CDR3 were randomized, contains sulfotyrosine and binds gp120 more effectively than a similarly displayed known sulfated antibody isolated from human serum. These experiments suggest that an expanded "synthetic" genetic code can confer a selective advantage in the directed evolution of proteins with specific properties.
Proceedings of the National Academy of Sciences of the United States of America © 2008 National Academy of Sciences