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Allelic Polymorphism and Transassociation of Molecules Encoded by the HLA-DQ Subregion
Robert C. Giles, Robert DeMars, Cecile C. Chang and J. Donald Capra
Proceedings of the National Academy of Sciences of the United States of America
Vol. 82, No. 6 (Mar. 15, 1985), pp. 1776-1780
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/25501
Page Count: 5
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A monoclonal antibody, CC11.23, with monomorphic specificity predominantly for products of the HLA-DQ subregion, has been used to demonstrate primary structural variation among DQ molecules. Two cell lines of each haplotype (DR1-7) were radiolabeled with [3H]tyrosine. α and β chains were isolated from CC11.23-reactive preparations, and their amino-terminal tyrosine sequences were determined. Each DR haplotype (with the exception of DRw6) was found to express a distinct DQ molecule with a minimum of three allelic forms of the DQ α chain and five allelic forms of the DQ β chain. At the primary structural level, the locus for the DQ β chain appears to be as polymorphic as the locus for the DR β chain. Unlike the locus for the DR α chain (which is essentially nonpolymorphic), the locus for the DQ α chain was found to be polymorphic. Comparison of DQ molecules from two different heterozygous cell lines with those from homozygous cell lines revealed that in heterozygotes, DQ α chains from either allele can associate with DQ β chains from one allele. The formation of hybrid HLA-DQ molecules by both cis and trans gene complementation, coupled with several polymorphic forms of each of the DQ subunits, considerably increases the repertoire of DQ alloantigens in heterozygotes.
Proceedings of the National Academy of Sciences of the United States of America © 1985 National Academy of Sciences