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Allelic Polymorphism and Transassociation of Molecules Encoded by the HLA-DQ Subregion

Robert C. Giles, Robert DeMars, Cecile C. Chang and J. Donald Capra
Proceedings of the National Academy of Sciences of the United States of America
Vol. 82, No. 6 (Mar. 15, 1985), pp. 1776-1780
Stable URL: http://www.jstor.org/stable/25501
Page Count: 5
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Allelic Polymorphism and Transassociation of Molecules Encoded by the HLA-DQ Subregion
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Abstract

A monoclonal antibody, CC11.23, with monomorphic specificity predominantly for products of the HLA-DQ subregion, has been used to demonstrate primary structural variation among DQ molecules. Two cell lines of each haplotype (DR1-7) were radiolabeled with [3H]tyrosine. α and β chains were isolated from CC11.23-reactive preparations, and their amino-terminal tyrosine sequences were determined. Each DR haplotype (with the exception of DRw6) was found to express a distinct DQ molecule with a minimum of three allelic forms of the DQ α chain and five allelic forms of the DQ β chain. At the primary structural level, the locus for the DQ β chain appears to be as polymorphic as the locus for the DR β chain. Unlike the locus for the DR α chain (which is essentially nonpolymorphic), the locus for the DQ α chain was found to be polymorphic. Comparison of DQ molecules from two different heterozygous cell lines with those from homozygous cell lines revealed that in heterozygotes, DQ α chains from either allele can associate with DQ β chains from one allele. The formation of hybrid HLA-DQ molecules by both cis and trans gene complementation, coupled with several polymorphic forms of each of the DQ subunits, considerably increases the repertoire of DQ alloantigens in heterozygotes.

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