You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Quantification of Frost Damage in Plant Tissues by Rates of Electrolyte Leakage
M. B. Murray, J. N. Cape and D. Fowler
The New Phytologist
Vol. 113, No. 3 (Nov., 1989), pp. 307-311
Stable URL: http://www.jstor.org/stable/2557078
Page Count: 5
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
A method for assessing frost hardiness of plant tissues [using shoots of Picea rubens Sarg. syn P. rubra (Du Roi) Link] has been developed based upon the rate of electrolyte leakage from shoots immersed in distilled water after exposure to a range of freezing treatments. The relationship between conductivity (the electrolyte concentration in solution) and time has been shown to follow an asymptotic curve, which may be represented by a first-order equation: Ct - Co = (Cauto - Co) (1 - e-kt) where Ct is the conductivity at time t, Co is the initial conductivity, Cauto is the conductivity after autoclaving and k is the first-order rate constant (units time-1). The rate of electrolyte leakage (k) varies directly with the extent of tissue damage. In P. rubens a rate of 0.4% h-1 distinguished between shoots which eventually died, and shoots which remained alive. A minimum of 3 conductivity measurements (after 1 day, 5 days and after autoclaving) is required for a reliable estimate of k. This objective, quantitative method of assessing frost hardiness may therefore be used directly to estimate LT50 values within a population.
The New Phytologist © 1989 New Phytologist Trust