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Identification and Quantification of Trehalose in Vesicular-Arbuscular Mycorrhizal Fungi by In vivo 13C NMR and HPLC Analyses
G. Becard, L. W. Doner, D. B. Rolin, D. D. Douds and P. E. Pfeffer
The New Phytologist
Vol. 118, No. 4 (Aug., 1991), pp. 547-552
Stable URL: http://www.jstor.org/stable/2557582
Page Count: 6
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Natural abundance in vivo 13C NMR spectra were obtained from spores of three species of VA mycorrhizal fungi: Glomus intraradix Schenck and Smith, Glomus etunicatum Becker and Gerdemann, and Gigaspora margarita Becker and Hall. The presence of trehalose was established in the spectra of Gl. etunicatum and Gi. margarita. HPLC analyses supported these observations and indicated that Gl. intraradix spores also contained small amounts of trehalose, not readily detectable by 13C NMR. Trehalose constituted between 0·06% and 1·6% (w/w) of the spore dry weight, depending on the species. Only trace amounts of other sugars, including glycerol, were detected. An in vivo NMR time course experiment indicated that trehalose was readily utilized during spore germination.
The New Phytologist © 1991 New Phytologist Trust