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Freeze-Fracturing for Low-Temperature Scanning Electron Microscopy of Hartig Net in Synthesized Picea abies - Hebeloma crustuliniforme and - Tricholoma vaccinum Ectomycorrhizas

Christoph Scheidegger and Ivano Brunner
The New Phytologist
Vol. 123, No. 1 (Jan., 1993), pp. 123-132
Published by: Wiley on behalf of the New Phytologist Trust
Stable URL: http://www.jstor.org/stable/2557778
Page Count: 10
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Freeze-Fracturing for Low-Temperature Scanning Electron Microscopy of Hartig Net in Synthesized Picea abies - Hebeloma crustuliniforme and - Tricholoma vaccinum Ectomycorrhizas
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Abstract

Rapidly frozen ectomycorrhizal roots were freeze-fractured under high vacuum in a dedicated cryo-preparation unit and investigated in the frozen, partially freeze-dried, or fully hydrated state. Tangential, radial and occasional transverse surface views of Hartig net were obtained after freeze-fracturing. Protoplasmic and exoplasmic fracture faces of the fungal plasmalemma and occasional fractures between the fungal cell wall and the extracellular matrix were seen. Primary pit-fields with plasmodesmata occurred in non-ectomycorrhizal parts of the root as well as in small gaps of Hartig net. Septa with dolipores were observed in the finger-like complexes of the Hartig net.

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