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Stimulation by Auxin and Sucrose of Bulbil Formation In vitro by Single Leaf Cultures of Narcissus
I. Staikidou, C. Selby and B. M. R. Harvey
The New Phytologist
Vol. 127, No. 2 (Jun., 1994), pp. 315-320
Stable URL: http://www.jstor.org/stable/2558031
Page Count: 6
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Bulbil formation in vitro by single leaf explants from shoot clump cultures was investigated for Narcissus cultivars St Keverne and Hawera. Single leaf explants (30-50 mm leaf with attached basal plate tissue) formed bulbils very infrequently unless cultured on media containing both auxin and a high concentration of sucrose whereas auxin was not necessary for bulbil formation by shoot clump cultures. As well as showing much stronger stimulation by auxin, bulbil initiation and development were more strongly inhibited by BA in single leaf cultures than in shoot clump cultures. Partitioning of dry matter into bulbils was generally lower in single leaf cultures than in shoot clump cultures but was more sensitive to growth regulators. Optimal sucrose and auxin concentrations for bulbil development by single leaf cultures were investigated. Bulbil formation in the presence of NAA was strongly stimulated by 176 mM sucrose for both cultivars but there were genotypic differences in auxin responses, St Keverne showing good bulbil development with 0.54 μM NAA, 5.4 μM IAA and 5.4 μM IBA and Hawera responding only to 27 μM IAA. It is concluded that single leaf cultures could provide a simple system for investigation of the regulation of Narcissus bulb initiation and development.
The New Phytologist © 1994 New Phytologist Trust