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Methionine Oxidation Contributes to Bacterial Killing by the Myeloperoxidase System of Neutrophils
Henry Rosen, Seymour J. Klebanoff, Yi Wang, Nathan Brot, Jay W. Heinecke and Xiaoyun Fu
Proceedings of the National Academy of Sciences of the United States of America
Vol. 106, No. 44 (Nov. 3, 2009), pp. 18686-18691
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/25593073
Page Count: 6
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Reactive oxygen intermediates generated by neutrophils kill bacteria and are implicated in inflammatory tissue injury, but precise molecular targets are undefined. We demonstrate that neutrophils use myeloperoxidase (MPO) to convert methionine residues of ingested Escherichia coli to methionine sulfoxide in high yield. Neutrophils deficient in individual components of the MPO system (MPO, H₂O₂, chloride) exhibited impaired bactericidal activity and impaired capacity to oxidize methionine. HOCl, the principal physiologic product of the MPO system, is a highly efficient oxidant for methionine, and its microbicidal effects were found to correspond linearly with oxidation of methionine residues in bacterial cytosolic and inner membrane proteins. In contrast, outer envelope proteins were initially oxidized without associated microbicidal effect. Disruption of bacterial methionine sulfoxide repair systems rendered E. coli more susceptible to killing by HOCl, whereas over-expression of a repair enzyme, methionine sulfoxide reductase A, rendered them resistant, suggesting a direct role for methionine oxidation in bactericidal activity. Prominent among oxidized bacterial proteins were those engaged in synthesis and translocation of peptides to the cell envelope, an essential physiological function. Moreover, HOCl impaired protein translocation early in the course of bacterial killing. Together, our findings indicate that MPO-mediated methionine oxidation contributes to bacterial killing by neutrophils. The findings further suggest that protein translocation to the cell envelope is one important pathway targeted for damage.
Proceedings of the National Academy of Sciences of the United States of America © 2009 National Academy of Sciences