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Functional Modules in the Arabidopsis Core Cell Cycle Binary Protein—Protein Interaction Network

Joanna Boruc, Hilde Van den Daele, Jens Hollunder, Stephane Rombauts, Evelien Mylle, Pierre Hilson, Dirk Inzé, Lieven De Veylder and Eugenia Russinova
The Plant Cell
Vol. 22, No. 4 (APRIL 2010), pp. 1264-1280
Stable URL: http://www.jstor.org/stable/25680131
Page Count: 17
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Functional Modules in the
              Arabidopsis
              Core Cell Cycle Binary Protein—Protein Interaction Network
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Abstract

As in other eukaryotes, cell division in plants is highly conserved and regulated by cyclin-dependent kinases (CDKs) that are themselves predominantly regulated at the posttranscriptional level by their association with proteins such as cyclins. Although over the last years the knowledge of the plant cell cycle has considerably increased, little is known on the assembly and regulation of the different CDK complexes. To map protein—protein interactions between core cell cycle proteins of Arabidopsis thaliana, a binary protein—protein interactome network was generated using two complementary high-throughput interaction assays, yeast two-hybrid and bimolecular fluorescence complementation. Pairwise interactions among 58 core cell cycle proteins were tested, resulting in 357 interactions, of which 293 have not been reported before. Integration of the binary interaction results with cell cycle phase-dependent expression information and localization data allowed the construction of a dynamic interaction network. The obtained interaction map constitutes a framework for further in-depth analysis of the cell cycle machinery.

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