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Five Arabidopsis Reticulon Isoforms Share Endoplasmic Reticulum Location, Topology, and Membrane-Shaping Properties
Imogen Sparkes, Nicholas Tolley, Isabel Aller, Julia Svozil, Anne Osterrieder, Stanley Botchway, Christopher Mueller, Lorenzo Frigerio and Chris Hawes
The Plant Cell
Vol. 22, No. 4 (APRIL 2010), pp. 1333-1343
Published by: American Society of Plant Biologists (ASPB)
Stable URL: http://www.jstor.org/stable/25680135
Page Count: 11
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The cortical endoplasmic reticulum (ER) in tobacco (Nicotiana tabacum) epidermal cells is a network of tubules and cisternae undergoing dramatic rearrangements. Reticulons are integral membrane proteins involved in shaping ER tubules. Here, we characterized the localization, topology, effect, and interactions of five Arabidopsis thaliana reticulons (RTNs), isoforms 1-4 and 13, in the cortical ER. Our results indicate that RTNLB13 and RTNLB1-4 colocate to and constrict the tubular ER membrane. All five RTNs preferentially accumulate on ER tubules and are excluded from ER cisternae. All isoforms share the same transmembrane topology, with N and C termini facing the cytosol and four transmembrane domains. We show by Förster resonance energy transfer and fluorescence lifetime imaging microscopy that several RTNs have the capacity to interact with themselves and each other, and we suggest that oligomerization is responsible for their residence in the ER membrane. We also show that a complete reticulon homology domain is required for both RTN residence in high-curvature ER membranes and ER tubule constriction, yet it is not necessary for homotypic interactions.
The Plant Cell © 2010 American Society of Plant Biologists (ASPB)