Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Arabidopsis Basic Leucine-Zipper Transcription Factors TGA9 and TGA10 Interact with Floral Glutaredoxins ROXY1 and ROXY2 and Are Redundantly Required for Anther Development

Jhadeswar Murmu, Michael J. Bush, Catherine DeLong, Shutian Li, Mingli Xu, Madiha Khan, Caroline Malcolmson, Pierre R. Fobert, Sabine Zachgo and Shelley R. Hepworth
Plant Physiology
Vol. 154, No. 3 (November 2010), pp. 1492-1504
Stable URL: http://www.jstor.org/stable/25758692
Page Count: 13
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Arabidopsis Basic Leucine-Zipper Transcription Factors TGA9 and TGA10 Interact with Floral Glutaredoxins ROXY1 and ROXY2 and Are Redundantly Required for Anther Development
Preview not available

Abstract

ROXY1 and ROXY2 are CC-type floral glutaredoxins with redundant functions in Arabidopsis (Arabidopsis thaliana) anther development. We show here that plants lacking the basic leucine-zipper transcription factors TGA9 and TGA10 have defects in male gametogenesis that are strikingly similar to those in roxy1 roxy2 mutants. In tga9 tga10 mutants, adaxial and abaxial anther lobe development is differentially affected, with early steps in anther development blocked in adaxial lobes and later steps affected in abaxial lobes. Distinct from roxy1 roxy2, microspore development in abaxial anther lobes proceeds to a later stage with the production of inviable pollen grains contained within nondehiscent anthers. Histological analysis shows multiple defects in the anther dehiscence program, including abnormal stability and lignification of the middle layer and defects in septum and stomium function. Compatible with these defects, TGA9 and TGA10 are expressed throughout early anther primordia but resolve to the middle and tapetum layers during meiosis of pollen mother cells. Several lines of evidence suggest that ROXY promotion of anther development is mediated in part by TGA9 and TGA10. First, TGA9 and TGA10 expression overlaps with ROXY1/2 during anther development. Second, TGA9/10 and ROXY1/2 operate downstream of SPOROCYTELESS/NOZZLE, where they positively regulate a common set of genes that contribute to tapetal development. Third, TGA9 and TGA10 directly interact with ROXY proteins in yeast and in plant cell nuclei. These findings suggest that activation of TGA9/10 transcription factors by ROXY-mediated modification of cysteine residues promotes anther development, thus broadening our understanding of how redox-regulated TGA factors function in plants.

Page Thumbnails

  • Thumbnail: Page 
1492
    1492
  • Thumbnail: Page 
1493
    1493
  • Thumbnail: Page 
1494
    1494
  • Thumbnail: Page 
1495
    1495
  • Thumbnail: Page 
1496
    1496
  • Thumbnail: Page 
1497
    1497
  • Thumbnail: Page 
1498
    1498
  • Thumbnail: Page 
1499
    1499
  • Thumbnail: Page 
1500
    1500
  • Thumbnail: Page 
1501
    1501
  • Thumbnail: Page 
1502
    1502
  • Thumbnail: Page 
1503
    1503
  • Thumbnail: Page 
1504
    1504